Enhancing functional production of G protein‐coupled receptors in Pichia pastoris to levels required for structural studies via a single expression screen N André, N Cherouati, C Prual, T Steffan, G Zeder‐Lutz, T Magnin, ... Protein Science 15 (5), 1115-1126, 2006 | 225 | 2006 |
Structural genomics on membrane proteins: comparison of more than 100 GPCRs in 3 expression systems K Lundstrom, R Wagner, C Reinhart, A Desmyter, N Cherouati, T Magnin, ... Journal of structural and functional genomics 7, 77-91, 2006 | 157 | 2006 |
Detergent-free isolation of functional G protein-coupled receptors into nanometric lipid particles C Logez, M Damian, C Legros, C Dupré, M Guéry, S Mary, R Wagner, ... Biochemistry 55 (1), 38-48, 2016 | 110 | 2016 |
Semliki Forest virus vectors for overexpression of 101 G protein-coupled receptors in mammalian host cells G Hassaine, R Wagner, J Kempf, N Cherouati, N Hassaine, C Prual, ... Protein expression and purification 45 (2), 343-351, 2006 | 88 | 2006 |
Quantification of detergents complexed with membrane proteins V Chaptal, F Delolme, A Kilburg, S Magnard, C Montigny, M Picard, ... Scientific reports 7 (1), 41751, 2017 | 75 | 2017 |
Isolation and pharmacological characterization of AdTx1, a natural peptide displaying specific insurmountable antagonism of the α1A‐adrenoceptor L Quinton, E Girard, A Maiga, M Rekik, P Lluel, G Masuyer, M Larregola, ... British journal of pharmacology 159 (2), 316-325, 2010 | 66 | 2010 |
GHSR-D2R heteromerization modulates dopamine signaling through an effect on G protein conformation M Damian, V Pons, P Renault, C M’kadmi, B Delort, L Hartmann, AI Kaya, ... Proceedings of the National Academy of Sciences 115 (17), 4501-4506, 2018 | 65 | 2018 |
Large-scale functional expression of WT and truncated human adenosine A2A receptor in Pichia pastoris bioreactor cultures S Singh, A Gras, C Fiez-Vandal, J Ruprecht, R Rana, M Martinez, ... Microbial cell factories 7, 1-10, 2008 | 61 | 2008 |
Mammalian G protein-coupled receptor expression in Escherichia coli: II. Refolding and biophysical characterization of mouse cannabinoid receptor 1 and human parathyroid … K Michalke, C Huyghe, J Lichière, ME Gravière, M Siponen, G Sciara, ... Analytical biochemistry 401 (1), 74-80, 2010 | 58 | 2010 |
Expression of EGFP-amino-tagged human mu opioid receptor in Drosophila Schneider 2 cells: a potential expression system for large-scale production of G-protein coupled receptors BG Perret, R Wagner, S Lecat, K Brillet, G Rabut, B Bucher, F Pattus Protein expression and purification 31 (1), 123-132, 2003 | 52 | 2003 |
Mammalian G-protein-coupled receptor expression in Escherichia coli: I. High-throughput large-scale production as inclusion bodies K Michalke, ME Gravière, C Huyghe, R Vincentelli, R Wagner, F Pattus, ... Analytical biochemistry 386 (2), 147-155, 2009 | 48 | 2009 |
Dot-blot immunodetection as a versatile and high-throughput assay to evaluate recombinant GPCRs produced in the yeast Pichia pastoris G Zeder-Lutz, N Cherouati, C Reinhart, F Pattus, R Wagner Protein expression and purification 50 (1), 118-127, 2006 | 43 | 2006 |
Identification of a novel protein-protein interaction motif mediating interaction of GPCR-associated sorting proteins with G protein-coupled receptors O Bornert, TC Møller, J Boeuf, MP Candusso, R Wagner, KL Martinez, ... PloS one 8 (2), e56336, 2013 | 41 | 2013 |
From purified GPCRs to drug discovery: the promise of protein-based methodologies F Alkhalfioui, T Magnin, R Wagner Current opinion in pharmacology 9 (5), 629-635, 2009 | 41 | 2009 |
The ORF YBL042 of Saccharomyces cerevisiae encodes a uridine permease R Wagner, J De Montigny, P De Wergifosse, JL Souciet, S Potier FEMS microbiology letters 159 (1), 69-75, 1998 | 39 | 1998 |
Increasing cell biomass in Saccharomyces cerevisiae increases recombinant protein yield: the use of a respiratory strain as a microbial cell factory C Ferndahl, N Bonander, C Logez, R Wagner, L Gustafsson, C Larsson, ... Microbial cell factories 9, 1-11, 2010 | 38 | 2010 |
Welding fume nanoparticles from solid and flux-cored wires: Solubility, toxicity, and role of fluorides YS Hedberg, Z Wei, S McCarrick, V Romanovski, J Theodore, EM Westin, ... Journal of Hazardous Materials 413, 125273, 2021 | 35 | 2021 |
Overexpression of Membrane Proteins Using Pichia pastoris O Bornert, F Alkhalfioui, C Logez, R Wagner Current protocols in protein science 29 (1), 29.2. 1-29.2. 24, 2002 | 33 | 2002 |
Quantitative RT-PCR for titration of replication-defective recombinant Semliki Forest virus ALP Puglia, AG Rezende, SAC Jorge, R Wagner, CA Pereira, RM Astray Journal of virological methods 193 (2), 647-652, 2013 | 27 | 2013 |
Recombinant human melatonin receptor MT1 isolated in mixed detergents shows pharmacology similar to that in mammalian cell membranes C Logez, S Berger, C Legros, JL Baneres, W Cohen, P Delagrange, ... PLoS One 9 (6), e100616, 2014 | 26 | 2014 |